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【目的】为了明确解毒酶对绿盲蝽Apolygus lucorum防治药剂温度效应的影响机制。【方法】本文测定了绿盲蝽3龄若虫体内3种主要解毒酶活力随温度变化的“钟形”曲线,以及在不同温度效应杀虫剂诱导后对绿盲蝽体内解毒酶“钟形”曲线的影响。【结果】在供试温度10~40℃范围内,绿盲蝽体内3种主要解毒酶,在25℃时活力均最高,且显著高于其他温度处理。在不同温度下,4种不同温度效应杀虫剂诱导后对绿盲蝽体内解毒酶活力影响测定结果来看,谷胱甘肽-S-转移酶受杀虫剂诱导影响,可能参与了负温度效应杀虫剂溴虫腈的代谢,其在20~30℃的温度范围内,可能参与了吡虫啉和辛硫磷的部分代谢,而正温度效应杀虫剂氟铃脲在低温时毒力较低,可能与其诱导该酶在低温时活力升高相关。在15℃左右的低温时,绿盲蝽体内羧酸酯酶和多功能氧化酶易受氟铃脲和溴虫腈诱导而活力增加,而在20~35℃,这两种酶活力不易受杀虫剂诱导,且辛硫磷、溴虫腈和吡虫啉可对绿盲蝽体内两种酶活力产生一定的抑制作用。【结论】绿盲蝽体内解毒酶活力可明显受到温度影响,而在杀虫剂诱导条件下,其可对绿盲蝽防治药剂的温度效应产生显著影响。
【Objective】 To clarify the mechanism of detoxification enzyme’s effect on temperature effect of Apolygus lucorum. 【Method】 The “bell-shaped” curve of the activity changes of three main detoxification enzymes in the 3rd instar nymphs of Lygus nudibranch was studied in this paper. The “dementia” Bell-shaped "curve of the impact. 【Result】 The results showed that in the temperature range of 10 ~ 40 ℃, the three main detoxification enzymes in the body of M. lucorum had the highest activity at 25 ℃, which was significantly higher than that of other temperature treatments. At different temperatures, four different temperature-effect insecticides induced detoxification enzyme activity of green-smoked stinkbug in vivo determination results, glutathione-S-transferase insecticide-induced effects may be involved in the negative temperature The effect of insecticide chlorfenapyr metabolism, which may be involved in the partial metabolism of imidacloprid and phoxim, in the temperature range of 20-30 ° C, while the positive temperature-effect insecticide hexaflumuron is less virulent at low temperatures , May be related to its induction of increased activity of the enzyme at low temperatures. At low temperature around 15 ℃, carboxylesterase and polyfunctional oxidase in green houseplants were more susceptible to hexaflumuron and chlorfenapyr induced increase in vitality, while at 20 ~ 35 ℃, these two enzyme activities are not easy to kill Induced by insecticides, and phoxim, chlorfenapyr and imidacloprid could inhibit the activity of two kinds of enzymes in the green-houseworm. 【Conclusion】 The activity of detoxification enzyme in M. lucorum was significantly affected by temperature. Under insecticide-induced conditions, it could have a significant effect on the temperature effect of M. lucorum.