论文部分内容阅读
目的 :探讨腺苷脱氨酶 (ADA)、鸟嘌呤酶 (GUA)、IL -6和 IL-8,在庚型肝炎病毒 (HGV)复制中的改变。方法 :以RT-PCR分析不同肝病患者中 HGV-RNA,并对反映肝细胞损害灵敏的嘌呤酶活性、IL -6和 IL -8浓度进行检测 ,对单纯 HGV感染和 HGV与其它病毒复合感染作了比较分析。结果 :在肝病组中 HGV-RNA阳性率为 10 .5 % ,在非肝病组中的阳性率为 7.5 %。在 37例 HGV-RNA阳性患者中 ,HBV-DNA阳性占 35 .5 % ,HCV-RNA阳性占 16 .2 % ,三种病毒同时阳性占 5 .4%。在 HGV患者中 ,AL T活性异常占 89.2 % ,ADA活性异常占 70 .3% ,GUA活性异常占 45 .9%。庚型肝炎患者 HGV复制时 ,血清 IL -6和 IL-8水平明显高于非肝病组患者 ,在 HGV与其它病毒复合感染时 ,IL-6和 IL-8浓度显著升高。结论 :嘌呤酶是反映 HGV复制的有用标志 ,IL-6和 IL-8可能参与肝细胞损伤的炎症过程
Objective: To investigate the changes of adenosine deaminase (ADA), guanosine (GUA), IL-6 and IL-8 in the replication of hepatitis G virus (HGV). Methods: HGV-RNA was analyzed by RT-PCR in patients with different liver diseases. The activities of purine enzymes, IL-6 and IL-8 were detected. The activities of pure HGV and HGV combined with other viruses A comparative analysis. Results: The positive rate of HGV-RNA was 10.5% in liver disease group and 7.5% in non-liver disease group. Among 37 HGV-RNA positive patients, HBV-DNA positive accounted for 35.5%, HCV-RNA positive accounted for 16.2%, three viruses simultaneously positive accounted for 5.4%. In HGV patients, ALT activity accounted for 89.2%, ADA activity was 70.3%, GUA activity was 45.9%. In HGV patients with GGV infection, the levels of IL-6 and IL-8 in serum were significantly higher than those in non-liver disease patients. IL-6 and IL-8 concentrations were significantly increased when HGV was co-infected with other viruses. Conclusion: Purine is a useful marker of HGV replication. IL-6 and IL-8 may be involved in the inflammatory process of hepatocyte injury