论文部分内容阅读
（目的〕了解分子生物学检测方法是否对肺癌的诊断具有辅助价值。（方法）痰液处理方法为痰液0．5毫升加入痰处理液制备细胞沉淀液，酚氯仿提取DNA；SSCP－PCR银染和RFLP－PCR方法对痰液中P53、K－ras突变情况进行检测。（结果）在确诊的76例肺癌病人中，p53突变率为40．8％，K－ras突变率为25．0％，突变与肺癌类型、分期未见明显相关，其中有29例病人痰液普通脱落细胞学检测明性．痰液p53或K－ras突变阳性，最后经纤支镜刷检，活检部分经过手术征实为肺癌，有12例经手术证实为Ⅰ～Ⅱ期的相对早期的肺癌。(结论)痰液SSCP－PCR、RELP-PCR方法检测癌基因突变具有简便、实用、准确、可行的特点。对部分疑为肺癌而痰液脱落细胞学检测阴性的病人具有一定的辅助诊断价值。
(Objective) To understand whether the molecular biological detection method is useful for the diagnosis of lung cancer. (Methods) Sputum solution 0.5 ml of sputum was added to the sputum solution to prepare cell pellets, and phenol-chloroform was used to extract DNA; SSCP-PCR silver Staining and RFLP-PCR methods were used to detect mutations in P53 and K-ras in sputum.(Results) Among the 76 lung cancer patients diagnosed, the mutation rate of p53 was 40.8%, and the mutation rate of K-ras was 25.0. %, Mutations were not significantly associated with the type and staging of lung cancer. Among them, 29 patients had sputum with normal shedding cytology and sputum positive for p53 or K-ras mutations. Finally, fibrobronchoscopy was performed and the biopsy was operated on In the case of lung cancer, there were 12 relatively early stage lung cancers that were confirmed to be stage I to II by surgery.(Conclusion) Sputum SSCP-PCR and RELP-PCR methods are simple, practical, accurate, and feasible for detecting oncogene mutations. For some patients suspected of having lung cancer and sputum cytology negative test, it has certain auxiliary diagnostic value.