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[目的]研究肿瘤抗原致敏的树突状细胞(DC)诱导淋巴因子激活的杀伤细胞(LAK)和细胞因子诱导的杀伤细胞(CIK)对肺癌细胞株A549和肺腺癌原代细胞的杀伤作用。[方法]取健康人外周血单个核细胞,常规诱导出DC、CIK、LAK细胞。用肺癌A549细胞提取的肿瘤抗原冲击DC,倒置显微镜下观察DC形态。流式细胞仪检测DC经抗原冲击和未经抗原冲击后其表型变化。LDH释放法测定杀伤活性。[结果]DC经肿瘤抗原冲击后在镜下呈典型成熟形态,其表面分子CD40、CD80、CD86和HLA-DR的表达明显较未经肿瘤抗原冲击的DC高。DC+CIK细胞对A549和肺腺癌原代细胞的杀伤活性高于CIK细胞、LAK细胞和DC+LAK细胞(P<0.05),随着效靶比的升高,其杀伤效应随之增强(P<0.05)。[结论]肿瘤抗原致敏的DC可诱导特异性CIK细胞,DC+CIK细胞对A549和肺腺癌原代细胞的杀伤作用明显高于DC+LAK、CIK、LAK细胞。
[Objective] To study the killing effect of tumor antigen-primed dendritic cells (DCs) induced lymphokine-activated killer cells (LAKs) and cytokine-induced killer cells (CIKs) on lung cancer cell lines A549 and primary lung adenocarcinoma cells effect. [Methods] Peripheral blood mononuclear cells from normal people were induced and DC, CIK and LAK cells were routinely induced. Tumor antigens extracted from A549 lung cancer cells were challenged with DC and the DC morphology was observed under an inverted microscope. Flow cytometry was used to detect the phenotypic changes of DCs after being challenged by antigen and without antigen. LDH release assay for killing activity. [Results] The DCs showed a typical mature morphology under the microscope after being impacted by tumor antigens. The expression of surface molecules CD40, CD80, CD86 and HLA-DR were significantly higher than those without tumor antigen challenge. The killing activity of DC + CIK cells on A549 and primary lung adenocarcinoma cells was higher than that of CIK cells, LAK cells and DC + LAK cells (P <0.05) P <0.05). [Conclusion] DCs primed by tumor antigen can induce specific CIK cells. The cytotoxicity of DC + CIK cells on A549 and primary lung adenocarcinoma cells was significantly higher than that of DC + LAK, CIK and LAK cells.