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目的:研究人唾液腺腺样囊性癌组织中MAPK(p-ERK1/2、p-P38及p-JNK)、MMP-2和MMP-9的表达情况,探讨其与临床病理特征的关系。方法:应用免疫组织化学SP法检测27例腺样囊性癌组织标本(癌组)和15例正常唾液腺组织标本(对照组)中p-ERK1/2、p-P38、p-JNK、MMP-2和MMP-9的表达情况。采用SPSS17.0软件包对数据进行统计学分析。结果:p-ERK1/2、p-P38、p-JNK、MMP-2和MMP-9在腺样囊性癌组中的阳性表达率(分别为81.48%、88.89%、70.37%、81.49%和77.78%)均显著高于在正常唾液腺对照组中的阳性表达率(分别为20%、26.67%、26.67%、26.67%和26.67%),差异有统计学意义(分别为P<0.01、P<0.01、P<0.05、P<0.01和P<0.01)。腺样囊性癌组织中p-ERK1/2、p-P38、MMP-2和MMP-9在Ⅲ～Ⅳ期组的阳性表达率(分别为88.89%、100%、94.44%和94.44%)显著高于Ⅰ～Ⅱ期组(分别为44.44%、66.67%、55.56%和44.44%),差异有统计学意义(分别为P<0.01、P<0.01、P<0.01和P<0.05),但p-JNK的表达与临床分期无统计学关系(P>0.05);p-ERK1/2、p-P38、p-JNK、MMP-2和MMP-9的表达与患者的性别、年龄、原发肿瘤部位、病理分型以及有无神经侵袭、复发和转移亦均无相关性(均为P>0.05);p-ERK1/2、p-P38、p-JNK与MMP-2和MMP-9的表达分别呈显著正相关(分别为r=0.786,P<0.001;r=0.796,P<0.001;r=0.824,P<0.001和r=0.768,P<0.001;r=0.581,P<0.01;r=0.604,P<0.01)。结论:p-ERK1/2、p-P38、p-JNK、MMP-2和MMP-9的高表达与腺样囊性癌的临床分期及进展密切相关。MAPK/MMPs通路可能参与腺样囊性癌的发生、发展、侵袭及转移过程。
OBJECTIVE: To investigate the expression of MAPK (p-ERK1 / 2, p-P38 and p-JNK), MMP-2 and MMP-9 in human salivary adenoid cystic carcinoma and to investigate its relationship with clinicopathological features. Methods: The expressions of p-ERK1 / 2, p-P38, p-JNK and MMP-9 in 27 cases of adenoid cystic carcinoma and 15 cases of normal salivary gland were detected by immunohistochemical SP method. 2 and MMP-9 expression. SPSS17.0 software package for statistical analysis of the data. Results: The positive rates of p-ERK1 / 2, p-P38, p-JNK, MMP-2 and MMP-9 in adenoid cystic carcinoma were 81.48%, 88.89%, 70.37%, 81.49% 77.78%) were significantly higher than those in the normal salivary gland control group (20%, 26.67%, 26.67%, 26.67% and 26.67%, respectively), the difference was statistically significant (P <0.01, P < 0.01, P <0.05, P <0.01 and P <0.01). The positive expression rates of p-ERK1 / 2, p-P38, MMP-2 and MMP-9 in group Ⅲ ~ Ⅳ of adenoid cystic carcinoma were 88.89%, 100%, 94.44% and 94.44% (P <0.01, P <0.01, P <0.01 and P <0.05, respectively), but the difference was statistically significant (P <0.01, P <0.01 and P < The expression of p-ERK1 / 2, p-P38, p-JNK, MMP-2 and MMP-9 had no statistical significance with the clinical stage (P> 0.05) The expression of p-ERK1 / 2, p-P38, p-JNK, MMP-2 and MMP-9 were also not related to the location, pathological type and the presence or absence of nerve invasion, recurrence and metastasis (R = 0.786, P <0.001; r = 0.796, P <0.001; r = 0.824, P <0.001 and r = 0.768, 0.604, P <0.01). Conclusion: The overexpression of p-ERK1 / 2, p-P38, p-JNK, MMP-2 and MMP-9 is closely related to the clinical stage and progression of adenoid cystic carcinoma. MAPK / MMPs pathway may be involved in the occurrence, development, invasion and metastasis of adenoid cystic carcinoma.