对我国分离的经生物学和血清学鉴定为黄瓜花叶病毒（ＣＭＶ）亚组Ⅰ和Ⅱ的各一分离物（ＧＢ、ＸＢ）的外壳蛋白（ＣＰ）基因进行了序列分析和比较。以提纯病毒ＲＮＡ为模板，进行逆转录及ＰＣＲ扩增，并通过常规基因克隆方法得到插入ＣＰ基因片段的重组克隆。对插入ＧＢ和ＸＢ两个分离物ＣＰ基因片段的重组克隆进行全序列测定，结果表明重组克隆序列长分别为７７７ｂｐ和７９２ｂｐ，均只含一个开放读框（ＯＲＦ），长度为６５７ｎｔ，可编码２１８个氨基酸；两个分离物的ＣＰ基因核苷酸序列同源率为７７５％，氨基酸序列同源率为８２６％。与我国已报道的７个ＣＭＶ分离物的ＣＰ基因序列比较，分离物ＧＢ的同源率为９１３％～９７３％，分离物ＸＢ的同源率为７６６％～７８４％。与国际上已报道部分ＣＭＶ株系的ＣＰ基因序列相比较，分离物ＧＢ与亚组Ⅰ株系有更密切的亲缘关系，而分离物ＸＢ则与亚组Ⅱ株系的亲缘关系更密切。 The results of sequence analysis and comparison of the CP genes isolated from our country by the methods of biology and serology showed that the coat protein (CP) of each isolate (GB, XB) of subgroups I and II of cucumber mosaic virus (CMV). The purified viral RNA was used as a template for reverse transcription and PCR amplification, and cloned into the CP gene fragment by conventional gene cloning method. The full-length sequence analysis of the recombinant clones inserted into the CP gene fragment of GB and XB isolates showed that the recombinant clones had 777bp and 792bp in length, each containing only one open reading frame (ORF) with a length of 657 nt and encoding 218 Amino acids. The CP gene nucleotide homology of two isolates was 775% and the amino acid sequence homology was 826%. Compared with the CP gene sequences of seven CMV isolates reported in China, the homologies of isolated GBs ranged from 913% to 973%, and the homologies of isolated XBs ranged from 766% to 784 %. Compared with the CP gene sequences of some CMV strains reported in the world, isolate GB has a closer genetic relationship with subgroup I strain, whereas isolates XB are more closely related to subgroup II strain.