用酶标葡萄球菌A 蛋白作ELISA 检测临床确诊、显凝试验为阳性的钩端螺旋体病人血清59份,同时用40份健康人血清与酶标抗体作ELISA进行比较。两种方法都有较高的敏感性和特异性,而酶标葡萄球菌A 蛋白具有制备简单,标记步骤简便,比较稳定和易被推广等优点,可替代酶标抗体在ELISA中应用。 Staphylococcus a protein by enzyme-linked ELISA test for clinical diagnosis, coagulation test positive for Leptospira patients serum 59, with 40 healthy human serum and ELISA antibody for ELISA comparison. Both methods have higher sensitivity and specificity, and Staphylococcus aureus protein A has the advantages of simple preparation, simple labeling steps, relatively stable and easy to be promoted, and can replace the use of ELISA antibody in ELISA.