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Objective: To explore the protective effect of minocycline on the apoptosis of cellular parkinsonism models induced by MPP+. Methods: Using PC12 cells as the apoptotic model of dopaminergic neurons, MC and MPP+ were added into the culture medium of PC12 cells, and using MTT to assay the cell viability and metabolic state; The cells apoptosis was assayed by electrophoresis method and using flow cytometry FACS to assay the apoptosis ratio. Results: Added the MPP+ to get the concentration of 10 μmol/L, the cellular parkinsonism model of apoptosis had been prepared. The pre-treatment of MC (100 μmol/L) could significantly increase the PC12 cell viability. The apoptosis ratio of MC+MPP+ group was significantly lower than that of MPP+ group, but was still significantly higher than that of control group. Conclusion: MC may protect the cell apoptosis induced by MPP+ to some extent.
Objective: To explore the protective effect of minocycline on the apoptosis of cellular parkinsonism models induced by MPP +. Methods: Using PC12 cells as the apoptotic model of dopaminergic neurons, MC and MPP + were added into the culture medium of PC12 cells, and using MTT to assay the cell viability and metabolic state; The cells apoptosis was assayed by electrophoresis method and using flow cytometry FACS to assay the apoptosis ratio. Results: Added the MPP + to get the concentration of 10 μmol / L, the cellular parkinsonism model of apoptosis had been The pre-treatment of MC (100 μmol / L) could significantly increase the PC12 cell viability. The apoptosis ratio of MC + MPP + group was significantly lower than that of MPP + group, but was still significantly higher than that of control group. Conclusion: MC may protect the cell apoptosis induced by MPP + to some extent.