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目的 探讨大鼠脑胶质瘤的形态学特征。方法 雄性SD大鼠 2 0只 ,体重 15 0～ 2 5 0克。立体定向注射 10 μl含有约 1× 10 6个C6细胞的混悬液于鼠脑右侧尾状核内 ,建立鼠脑胶质瘤模型。术后第 2 5天处死大鼠 ,立刻取脑 ,制作病理切片 ,HE及免疫组织化学染色 ,光镜下观察。结果 2 0只大鼠均有脑胶质瘤 ,其中星形细胞瘤Ⅱ级 (6只 )、星形细胞瘤Ⅲ级 (8只 )、星形细胞瘤Ⅳ级 (3只 )及少突胶质细胞瘤Ⅱ～Ⅳ级 (3只 )。经免疫组织化学染色检测S 10 0蛋白均为阳性。结论 C6细胞移植产生的鼠脑胶质瘤 ,其恶性程度不一致
Objective To investigate the morphological characteristics of glioma in rats. Methods Twenty male SD rats weighing 15 0-250 g were used. Stereotaxic injection of 10 μl of a suspension containing about 1 × 10 6 C6 cells in the right caudate nucleus of the rat brain established mouse glioma model. The rats were sacrificed on the 25th day after operation, and the brains were harvested immediately. Pathological sections were made and stained with HE and immunohistochemistry. Results Twenty gliomas were found in all 20 rats, including astrocytoma grade Ⅱ (6), astrocytoma grade Ⅲ (8), astrocytoma grade Ⅳ (3) and oligodendrocyte Stromal cell tumors Ⅱ ~ Ⅳ grade (3). Immunohistochemical staining S 10 0 protein were positive. Conclusion C6 glioma mouse glioma transplantation, the degree of malignancy is inconsistent