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目的：探讨PCR检测ALt－MRD的重要性及临床意义。方法：运用PCR技术，以IgH和TCRγ基因重排作为标志，检测了40例急性淋巴细胞白血病（ALL）微小残留病（MRD）。结果：7例初治未缓解，12例部分缓解（PR），13例完全缓解（CR）的ALL患儿MRD检测阳性，另8例（1例PR、7例CR）检测MRI）阴性。发现CR时间越长，MRD阳性率越低，反之，CR时间越短，MRD阳性率越高。结论：MRD可作为白血病治疗效果及判断预后的一个重要指标。CR后，MRD检测阳性有复发的危险；长期的MRD检测阴性，可作为临床停药观察的一个重要指标。PCR方法准确、先进，适于广泛推广应用。
Objective: To investigate the significance and clinical significance of PCR in detecting ALT-MRD. Methods: Forty cases of acute lymphoblastic leukemia (ALL) minimal residual disease (MRD) were detected by PCR using IgH and TCRγ gene rearrangements as markers. Results: Seven patients had no remission, 12 patients with partial remission (PR) and 13 patients with complete remission (CR). MRD was positive in 8 children (PR in 1 patient and CR in 7 patients). The longer the CR, the lower the MRD positive rate. On the contrary, the shorter the CR time, the higher the MRD positive rate. Conclusion: MRD can be used as an important indicator of the therapeutic effect and prognosis of leukemia. After the CR, MRD test positive for the risk of recurrence; long-term negative MRD test can be used as an important indicator of clinical withdrawal observed. PCR method is accurate, advanced, suitable for wide application.