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JWA, a cytoskeleton associated gene, was primarily found to be regulated by all trans-retinoic acid (ATRA), 13 cis-retinoic acid (13 cis-RA) and 12-tetradecano-ylphorbol-13-acetate (TPA). Our previous data showed that JWA might be involved in both cellular differentiation and apoptosis induced by several chemicals. In this study, we addressed the possible mechanism of JWA in the regulation of cell differentiation and apoptosis in NB4, a human acute promyelocytic leukemia cell line. CD11b/CD33 expression and cell cycle were analyzed for detecting of cell differentiation and apoptosis. Both reverse-transcription polymerase chain reaction (RT-PCR) and Western blot assays were used for understanding the expressions of JWA. The results showed that under the indicated concentrations ATRA (10-6 mol/L) and As2O3 (10-6 mol/L) induced cell differentiation and apoptosis separately; while both 4HPR (10-6mol/L) and TPA (10-7 mol/L) showed dual-directional effects on NB4 cells, they not only trigger cells’ differentia
JWA, a cytoskeleton associated gene, was was found to be regulated by all trans-retinoic acid (ATRA), 13 cis-retinoic acid (13 cis-RA) and 12-tetradecano- ylphorbol- 13-acetate data showed that JWA might be involved in both cellular differentiation and apoptosis induced by several chemicals. In this study, we addressed the possible mechanism of JWA in regulation of cell differentiation and apoptosis in NB4, a human acute promyelocytic leukemia cell line. CD11b / Both reverse-transcription polymerase chain reaction (RT-PCR) and Western blot assays were used for understanding the expressions of JWA. The results showed that under indicated indicated ATRA ( While both 2 HP (10-6 mol / L) and TPA (10-7 mol / L) showed dual-directional effects on NB4 cells, they not only trigger cells’ differentia