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通过菌丝对峙、双重培养,以及对发病双孢蘑菇子实体的显微观察,探讨有害疣孢霉菌Mycogone perniciosa(MP0012)与双孢蘑菇Agaricus bisporus(As2796)之间的互作关系。结果表明,在菌丝对峙生长阶段有害疣孢霉菌菌丝不侵入双孢蘑菇菌丝体内,两者可交叉生长,对双孢蘑菇生长影响不显著;对峙与双重培养均显示有害疣孢霉菌菌丝会产生对双孢蘑菇菌丝生长有抑制作用的挥发性物质,造成双孢蘑菇菌丝扭结断裂。同时试验证实了双孢蘑菇菌丝会促进有害疣孢霉菌厚垣孢子的产生和萌发、菌丝生长和发育。侵染实验结果表明,有害疣孢霉菌可直接侵染双孢蘑菇子实体,引起双孢蘑菇子实体病害;对罹病子实体显微观察结果发现,发病前期双孢蘑菇子实体表面长出绒毛状病原菌丝,菌柄中空,菌褶褐变腐烂并长出病原菌丝;发病中期双孢蘑菇子实体内菌丝组织会出现萎缩裂解现象,在近有害疣孢霉菌菌丝一侧的双孢蘑菇子实体菌丝细胞壁被降解;发病后期双孢蘑菇子实体菌丝组织基本消失。由此初步判断有害疣孢霉菌对双孢蘑菇的寄生类型偏向于死体营养型。
The interaction between mycogon perniciosa (MP0012) and Agaricus bisporus (As2796) was investigated by mycelial confrontation, double culture and microscopic observation of the fruiting body of Agaricus bisporus. The results showed that during the confrontation of mycelial growth phase, the mycelial growth of Mycosphaera tricolor was not invaded into the mycelium of Agaricus bisporus, and the growth of Agaricus bisporus was not significant. The antagonistic and dual culture showed that the mycoplasma noduleum Silk will have a inhibitory effect on the growth of Agaricus bisporus volatile substances, resulting in the mycelium twist of Agaricus bisporus rupture. At the same time, experiments confirmed that Agaricus bisporus mycelium will promote the generation and germination of mycelial chlamydospores, mycelium growth and development. The result of infection experiment showed that P. nidulans could directly infect Agaricus bisporus fruiting body and cause the Agaricus bisporus fruiting body disease. The microscopic observation on the diseased fruiting body showed that the early fruiting bodies of Agaricus bisporus grew villous Pathogenic mycelium, stipe stalk hollow, rotten follicular brown rot and grow the pathogenic mycelium; mid-term Agaricus bisporus fruiting body mycelium will appear shrinkage cracking phenomenon, near the side of the harmful verruca mycelium bisporus mushroom The mycelial cell wall of the mycelium was degraded; the mycelial structure of Agaricus bisporus fruiting bodies at the late stage of pathogenesis basically disappeared. From this, we initially determine that the parasitic type of A. nidulans against Agaricus bisporus is biased towards dead body nutrition.