OBJECTIVE Eriocalyxin B(EriB)is a natural diterpenoid purified fromIsodoneriocalyxvar.laxiflora,traditional Chinese herbal medicines,possesses strong antileukemic activity with low toxicity.In murine t(8;21)leukemia models,EriB remarkably prolong the survival time and decreased the xenograft tumor size by targeting AML1-ETO oncoprotein.In angiogenesis research by the highly vascularized chorioallantoic membrane(CAM)of the chicken embryo further confirm its antiangiogenic activity.Microarray offers a high efficient approach to study the gene expression profile treated by EriB,so as to provide systematic information about potential mechanisms of EriB curing AML.METHODS The t(8;21)AML cell line Kasumi-1 is most sensitive to EriB.Cells are treated with Eri-B(0.5μmol·L-1)and collected in 2,6,12 and 24h,respectively.Using human cDNA microarray,we investigate the changes of differential gene expression of Kasumi-1cells by time before and after Eri-B treatment.Meanwhile,the mRNA expression of TRAF2,DEDD2,BAG3,SAT1,IQGAP1,C-myc and GRB2 is detected by semi-quantitative RT-PCR and real-time quantitative PCR.RESULTS The genes regulated significantly are correlated with cell proliferation,apoptosis,cell circle,regulation of transcription,response to stimulies and metabolism.Regulation of TNFR mediated apoptotic signaling by EriB plays a key role to induce apoptosis and cell circle,involved NF-κB,Ras-MAPK,cAMP/PKA,PI3K/Akt,Cyt c/caspase and p53-Rb signal pathways.After detected by SqRT-PCR and real-time quantitative PCR,the mRNA expressions of BAG3,DEDD2,SAT1 and C-myc are significantly changed.CONCLUSION These findings describes the probable mechanisms involved and the value of EriB as a promising candidate targeting apoptosis cascade and cell circle in treatment of AML. OBJECTIVE Eriocalyxin B (EriB) is a natural diterpenoid purified fromIsodoneriocalyxvar.laxiflora, traditional Chinese herbal medicines, possesses strong antileukemic activity with low toxicity. Murine t (8; 21) leukemia models, EriB remarkably prolong the survival time and decreased the xenograft tumor size by targeting AML1-ETO oncoprotein. angiogenesis research by the highly vascularized chorioallantoic membrane (CAM) of the chicken embryo further confirm its antiangiogenic activity. Microarray offers a high efficient approach to study the gene expression profile treated by EriB, so as to provide System information about potential mechanisms of EriB curing AML. METHODS The t (8; 21) AML cell line Kasumi-1 is most sensitive to EriB.Cells are treated with Eri-B (0.5 μmol·L-1) and collected in 2, 6,12 and 24h, respectively. Using human cDNA microarray, we investigate the changes of differential gene expression of Kasumi-1 cells by time before and after Eri-B treatment. Meanwhile, the mRNA expression o f TRAF2, DEDD2, BAG3, SAT1, IQGAP1, C-myc and GRB2 were detected by semi-quantitative RT-PCR and real-time quantitative PCR.RESULTS The genes registered significantly correlated with cell proliferation, apoptosis, cell circle, regulation of transcription, response to stimulies and metabolism. Regulation of TNFR-mediated apoptotic signaling by EriB plays a key role to induce apoptosis and cell circle, involved in NF-κB, Ras-MAPK, cAMP / PKA, PI3K / Akt, Cyt c / caspase and p53 -Rb signal pathways. After detection by SqRT-PCR and real-time quantitative PCR, the mRNA expressions of BAG3, DEDD2, SAT1 and C-myc are significantly changed.CONCLUSION These findings describes the probable mechanisms involved and the value of EriB as a promising candidate targeting apoptosis cascade and cell circle in treatment of AML.