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目的观察内质网应激在染氟成骨细胞中的特征,探索氟能否直接引起成骨细胞内质网应激。方法 2.5、5、10、20、40、80mg/L氟化钠染毒原代培养成骨细胞,并设立空白对照组和衣霉素阳性对照组。24h后Western blot检测成骨细胞内重链结合蛋白(heavy-chain binding protein,BIP)、X盒结合蛋白-1(X box binging protein1,XBP-1)、生长抑制DNA损伤基因(CCAAT/enhancer-binding protein-homologous protein,CHOP,也称GADD153)、蛋白质二硫键异构酶(protein disulfideismoerase,PDI)表达水平。结果各染毒组PDI蛋白表达量与空白对照与比较差异无统计学意义,XBP-1从10mg/L氟化钠组起表达明显高于空白对照组,并随氟剂量表达量增加。从2.5mg/L氟化钠组起骨细胞中的BIP表达高于对照组(P<0.05);从10mg/L氟化钠组起,各氟染毒组的CHOP与β-actin的比值比对照组高(P<0.05)。结论氟能够引起成骨细胞内质网应激。
Objective To observe the characteristics of endoplasmic reticulum stress in fluoridated osteoblasts and to explore whether fluoride can directly cause endoplasmic reticulum stress in osteoblasts. Methods 2.5, 10, 20, 40, 80mg / L sodium fluoride primary culture of osteoblasts, and blank control group and tunicamycin positive control group. Western blot was used to detect the expression of BIP, XBP-1 and CCAAT / enhancer- binding protein-homologous protein (CHOP, also known as GADD153), protein disulfide isoerase (PDI) expression levels. Results There was no significant difference in the expression of PDI protein between the exposed groups and the blank control group. The expression of XBP-1 from the 10 mg / L sodium fluoride group was significantly higher than that of the blank control group, and increased with the increase of the fluoride dose. The expression of BIP in osteoblasts from 2.5mg / L sodium fluoride group was higher than that in control group (P <0.05). From 10mg / L sodium fluoride group, the ratio of CHOP to β-actin in each fluorine-exposed group was The control group was higher (P <0.05). Conclusion Fluorine can cause osteoporotic endoplasmic reticulum stress.