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为探讨微生物在红树林湿地生态系统中的作用,采用Biolog-ECO微平板技术分析东寨港红树林土壤微生物功能多样性在不同月份的变化特征.结果表明:红树林土壤微生物平均颜色变化率(AWCD)值12月份最高,4月份最低.Simpson指数、Shannon-Wiener指数和Pielou指数在2月份差异极显著.对6类碳源的利用差异显著(P<0.5),其中12月利用率最高,1月最低.氨基酸利用率最高,羧酸类利用率最低.主成分分析(PCA)表明,在31种因子种提取的2个主成分因子,分别可以解释所有方差变量的49.26%和22.26%,碳水化合物和氨基酸类为对PC1和PC2起分异作用的敏感碳源.红树林生态系统中,土壤微生物功能多样性随着时间尺度的变化而变化.说明红树林生态系统生物多样性丰富,蕴藏着丰富的微生物资源.
In order to explore the role of microorganisms in the mangrove wetland ecosystem, the Biolog-ECO microplate technique was used to analyze the variation of soil microbial functional diversity in different months in mangroves of Dongzhai Harbor.The results showed that the average color change rate of mangrove soil microbes AWCD) was the highest in December and the lowest in April, and the differences between Simpson index, Shannon-Wiener index and Pielou index were significant in February. There was significant difference (P <0.5) in the utilization of six carbon sources, of which the utilization rate was the highest in December, The lowest in January, the highest utilization of amino acids and the lowest utilization of carboxylic acids.The principal component analysis (PCA) showed that the two principal component factors extracted from 31 species could explain 49.26% and 22.26% of all variance respectively, Carbohydrates and amino acids were the sensitive carbon sources for the differentiation of PC1 and PC2. In mangrove ecosystem, the functional diversity of soil microbes changed with the time scale, indicating that the biodiversity of mangroves ecosystem is rich, With a wealth of microbial resources.