唐菖蒲(Gladiolus sp.)是一种颇受国内外人民欢迎的花卉,是各国花市上销路最广的切花之一。可是,由于唐菖蒲不耐高温,在南方种球常常退化,即使“南种北繁”也因种球繁殖系数较低远远满足不了国内外的需要。采用组织培养方法进行快速繁殖,对一些珍贵品种尤为必要。 1.材料与方法取唐菖蒲大球与子球为试材,剥去球体外部鳞片,冲洗干净,先用70％酒精浸泡1分钟,再在0.1％氯化汞溶液中浸15分钟充分消毒,用无菌水冲洗3次,把大球切成小 Gladiolus sp. Is a flower popular with people both at home and abroad. It is one of the most widely sold cut flowers in flower markets across the country. However, due to the glacial irrational high temperature, bulbs in the south often degenerate, even if the “southern species of North” because of the bulky coefficient of breeding is far less than the domestic and foreign needs. Tissue culture methods for rapid propagation of some precious species is particularly necessary. 1. Materials and methods Gladiolus balls and balls as the test material, stripped ball outer scales, rinse, first soaked with 70% alcohol for 1 minute, and then soaked in 0.1% mercuric chloride solution for 15 minutes to fully disinfect, Rinse 3 times with sterile water and cut the large ball into small pieces