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目的:探讨上皮性卵巢癌中环氧化酶-2(COX-2)的表达及临床意义。方法:采用链霉素抗生物素卵白素-过氧化物酶(SP)法进行免疫组化染色,观察COX-2在正常组织及良恶性上皮性卵巢癌组织中的蛋白表达,应用SPSS13.0软件结合病历资料进行分析。结果:正常组织及良恶性上皮性卵巢肿瘤中COX-2皆有表达,COX-2在卵巢癌组织中的阳性表达率78%(39/50)明显高于正常卵巢组织10%(1/10)及良性卵巢肿瘤组织20%(2/10),差异有统计学意义(P<0.01);在正常组织和良性卵巢肿瘤组织中的表达差异无统计学意义(P>0.05)。COX-2表达与病理分级及淋巴结转移明显相关(P<0.05),而与年龄、临床分期、组织学类型无关(P>0.05)。结论:COX-2在卵巢癌组织中的表达明显高于正常组织及良性卵巢肿瘤组织,在卵巢癌的浸润和转移中发挥了重要作用,可以作为判断卵巢癌恶性程度及评估不良预后的重要指标。
Objective: To investigate the expression of cyclooxygenase-2 (COX-2) in epithelial ovarian cancer and its clinical significance. Methods: Streptavidin-avidin-peroxidase (SP) method was used to detect the expression of COX-2 protein in normal tissues and benign and malignant epithelial ovarian cancer tissues. Immunohistochemical staining was performed by SPSS 13.0 Software combined with medical records data analysis. Results: The expression of COX-2 in both normal and benign and malignant ovarian tumors was significantly higher than that in normal ovarian tissues (78%, 39/50) ) And benign ovarian tumors (20%) (2/10), the difference was statistically significant (P <0.01). There was no significant difference in the expression between normal ovarian tissues and benign ovarian tumors (P> 0.05). COX-2 expression was significantly associated with pathological grade and lymph node metastasis (P <0.05), but not with age, clinical stage and histological type (P> 0.05). CONCLUSION: The expression of COX-2 in ovarian cancer tissues is significantly higher than that in normal tissues and benign ovarian tumor tissues, which plays an important role in the invasion and metastasis of ovarian cancer. It may be used as an important index for judging the malignancy and assessing the prognosis of ovarian cancer .