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以63份云南育种机构常用的甘蔗亲本资源为研究对象,10份甘蔗原始亲本种为外群体,使用30对多态性较高、在甘蔗遗传连锁图谱上分布较为广泛的Genomic-SSR引物对云南甘蔗常用亲本资源开展遗传多样性评价。结果表明:云南甘蔗常用亲本在30个SSR位点上表现出丰富的多态性,共获得363个扩增条带,其中多态性条带数为352个,平均多态性条带比例和多态性信息量分别为96.97%和0.9441;在Jaccard相似性系数方面,云南甘蔗常用亲本材料之间的相似性系数范围在0.2804~0.7329之间,平均为0.4309,表现出较大的遗传差异,其中来自大陆地区的亲本遗传差异最大,其次为来自国外地区的亲本;UPGMA聚类分析将所有亲本材料分为一大一小两个类群,而大类群又可分为2个亚类群,中国大陆地区一些既是主栽品种又是亲本的材料表现出较近的亲缘关系,而引自国外的大部分亲本和一些老的国内自育亲本也表现出较近的亲缘关系。以上结果将为上述亲本资源的高效利用奠定良好的基础,并为全国育种机构在亲本选择和组合配置上提供重要参考。
Sixty-three common sugarcane parental resources in breeding institutions in Yunnan Province were used as research objects. Ten of the original sugarcane-sugarcane parental plants were used as exosomes. Thirty pairs of Genomic-SSR primers with high polymorphism were used in Yunnan sugarcane genetic linkage map. Sugarcane commonly used parental resources to carry out genetic diversity assessment. The results showed that the common parents of Yunnan sugarcane showed rich polymorphism at 30 SSR loci, and a total of 363 amplified bands were obtained, in which the number of polymorphic bands was 352, the average polymorphic bands proportion and The amount of polymorphic information was 96.97% and 0.9441, respectively. For Jaccard similarity coefficient, the similarity coefficient of common parents in Yunnan sugarcane ranged from 0.2804 to 0.7329 with an average of 0.4309, indicating a large genetic difference. Among them, the parents from mainland China had the largest genetic differences, followed by those from other regions. UPGMA clustering analysis divided all the parents into one big, two small groups, while the large groups were divided into two subgroups. In the area, some cultivars and parents are closer relatives, while most of the parents from abroad and some older domestic self-bearing parents also show a closer relationship. The above results will lay a good foundation for the efficient utilization of the above-mentioned parental resources and provide an important reference for selection and combination allocation of parents in the nationwide breeding institutions.