Objective:This study evaluates the phytochemical constituents,antioxidant and anti-inflammatory activity,cytotoxicity,and inhibitory activity against carbohydrate metabolism of extracts from Ocotea bullata stem bark.Methods:Hexane,ethyl acetate,methanol and water were used to extract the air-dried sample.The phytochemical investigation and antioxidant assays were carried out on the extracts using standard procedures.The antidiabetic and anti-inflammatory potentials were evaluated using α-amylase,α-glucosidase and 5-lipoxygenase enzymes respectively.Veto cells were employed to determine the cytotoxicity of the extracts.Results:The ethyl acetate extract showed higher phenolic contents (8.97 mg/g gallic acid) while methanol displayed higher flavonoid (36.06 mg/g quercetin) and flavonol (153.44 mg/g turin) contents than other extracts.Hexane extract had the greatest capacity to scavenge 1,1-diphenyl-2-picryl-hydrazyl (0.19mg/mL),hydroxyl (25.77mg/mL) and 2,2-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid (0.07 mg/mL) radicals,while ethyl acetate extract exhibited stronger inhibition (P ＜ 0.05) against superoxide anion (0.41 mg/mL) and ferric ion-reducing power (2.36 mg/mL) compared to other extracts and standards.Aqueous extract (27.02 mg/mL) exhibited strong metal-chelating activity (P ＜ 0.05) compared to other extracts and gallic acid.The aqueous extract demonstrated the greatest inhibition of α-glucosidase (1.45 mg/mL) and α-amylase (2.43 mg/mL) compared to other extracts and acarbose.There were no significant differences (P ＜ 0.05) in half-maximum inhibitory concentration (ICso) values of all tested extracts and indomethacin in the inhibition of 5-lipoxygenase activity.The aqueous extract was nontoxic to Veto cells with an IC5o value of 0.38 mg/mL.Conclusion:O.bullata stem bark contains active phytochemicals with diverse pharmacological potentials that could be beneficial in managing diabetes and inflammation.