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背景:目前高钾心脏停搏液已在心脏手术中广泛应用。但有关高钾溶液对冠状动脉内皮细胞舒张血管功能影响的研究尚少。 目的:探讨高钾溶液对猪冠状动脉内皮细胞功能的影响及其机制。 设计:随机对照的实验研究。 地点、材料和干预:2000-06/09从北京大红门肉联厂共取17只刚宰杀的生猪猪心(雌雄不限)。均置入4℃的KH中,30min内送回北京阜外医院体外循环研究室,10min内选其心外膜下冠状动脉前降支中下三分之一,切成3段3mm的血管环。按单纯随机抽样法将49条猪冠状动脉血管环分为7组,每组7条;分别为对照组、20mmol/L高钾组、50mmol/L高钾组、四乙胺组、格列苯脲(商品名:优降糖)组、50mmol/L高钾+四乙胺组、50mmol/L高钾+格列苯脲组。采用器官槽法,分别用Krebs-Henseleit重碳酸盐缓冲液(Krebs solution, KH)、20,50mmol/L的高钾溶液浸泡血管环1h。 主要观察指标:7μmol/L环加氧酶阻断剂吲哚美辛(商品名:消炎痛)和300μmol/L一氧化氮合成酶阻断剂N-硝基-L-精氨酸、1mmol/L钙激动性钾通道阻断剂四乙胺或3μmol/L ATP敏感性钾通道阻断剂优降糖的作用下,30nmol/L前列腺素F_(2α)。引发的预收缩强度和非受体介导钙离子载体(1×10~(-10)～1×10~(-6)mol/L)引发的内皮源性舒张反应。 结果:格列苯脲组、50mmol/L高钾+四乙
Background: At present, high potassium cardioplegia is widely used in cardiac surgery. However, little research has been done on the effect of high potassium solution on vasodilatation of coronary artery endothelial cells. Objective: To investigate the effect of high potassium solution on the function of porcine coronary artery endothelial cells and its mechanism. Design: Randomized controlled experimental study. Venue, Materials and Intervention: A total of 17 heart-healthy pigs (male or female) were slaughtered from Beijing Dahongmen Meat Cohesion Factory on June 26, 2000. Were placed in KH 4 ℃, within 30min returned to Beijing Fuwai Hospital, Cardiopulmonary bypass laboratory, within 10min selected subendocardial anterior descending coronary artery in the lower third, cut into 3 sections of 3mm vascular ring . According to the method of simple random sampling, 49 swine coronary vessels were divided into 7 groups with 7 rats in each group. They were control group, 20 mmol / L potassium group, 50 mmol / L potassium group, tetraethylammonium group, Urea (trade name: glyburide) group, 50mmol / L potassium + tetraethylammonium group, 50mmol / L potassium + glibenclamide group. Using organ trough method, the vascular rings were immersed in Krebs-Henseleit Krebs solution (KH), 20, 50mmol / L potassium solution for 1h respectively. MAIN OUTCOME MEASURES: 7μmol / L cyclooxygenase inhibitor indomethacin (indomethacin) and 300μmol / L nitric oxide synthase inhibitor N-nitro-L-arginine, 1mmol / L calcium agonist potassium channel blocker tetraethylamine or 3 μmol / L ATP sensitive potassium channel blocker under the action of glyburide 30 nmol / L prostaglandin F 2α. Induced precondensation and endothelium - derived vasorelaxation induced by non - receptor - mediated calcium ionophore (1 × 10 -10 ~1 × 10 -6 mol / L). Results: Glibenclamide group, 50mmol / L potassium + tetrathia