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利用大白菜栽培品种的DH系Z16和白菜型油菜自交系L144杂交的F1获得包括119个株系的DH群体。用相同亲本的F1与Z16进行独立回交,利用25个独立回交的BC2分别得到这些株系的DH系,选择每个BC2的4～6个DH系构建121个株系的BIL群体。进一步利用SSR和SRAP标记构建了DH群体遗传图谱,由10个连锁群组成,包括245个分子标记,总长度714cM,平均遗传图距2.9cM。再以此图谱为参照,用锚定在染色体上的97个SSR标记研究供体亲本L144的染色体片段在BIL群体中覆盖基因组比率。在BIL群体中来源于L144的基因组片段占0.84%～35.00%,平均为11.31%,接近理论遗传预期值12.50%。在25个BC的BIL株系中供体亲本L144等位位点占1.69%～27.36%,平均为11.03%。
A DH population including 119 lines was obtained using F1 crossed with a Chinese cabbage cultivar DH line Z16 and a Brassica camouflage line L144. The independent parents were backcrossed with F1 and Z16 of the same parents. The DH lines of these lines were obtained by using 25 independent BC2 backcrosses. The BIL population of 121 lines was constructed with 4-6 DH lines of each BC2. The genetic map of DH population was further constructed using SSR and SRAP markers, and consisted of 10 linkage groups, including 245 molecular markers with a total length of 714cM and an average genetic distance of 2.9cM. Using this map as a reference, 97 SSR markers anchored on the chromosomes were used to study the coverage of genomic ratios in the BIL population by the chromosomal fragment of the donor parent L144. Genome fragments derived from L144 accounted for 0.84% -35.00% in the BIL population, with an average of 11.31%, close to the theoretical genetic prediction value of 12.50%. The donor parent L144 alleles accounted for 1.69% -27.36%, with an average of 11.03% in the 25 BC BIL lines.