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动物实验证实缺血性脑卒中与氧化应激有关。脑缺血或再灌注时,体内自由基生成增加,同时抗氯化物质水平下降,但此方面临床资料较少。本实验旨在研究缺血性卒中病人血浆抗氧化指标的变化及其与早期预后的关系。对象与方法发病24h内经CT确诊为缺血性脑卒中的38例病人作为研究对象,入院时及人院后第6、24h及第3、5、7d取血测定血浆维生素C、A、E、尿酸浓度及血浆中超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPX)活性,以及红细胞中SOD活性。血中维生素水平采用层析法测定,抗氧化酶活性采用分光光度法测定。病情严重程度及变化采用神经功能评分进行评价。取年龄和性别相匹配的37例健康人作为对照组。
Animal experiments confirmed that ischemic stroke and oxidative stress. Cerebral ischemia or reperfusion, the body increased free radicals, while the level of anti-chlorinated substances decreased, but less clinical data. The aim of this study was to investigate the changes of plasma antioxidant markers in patients with ischemic stroke and their relationship with early prognosis. Thirty-eight patients who were diagnosed as ischemic stroke by CT within 24 hours after onset of the disease were enrolled in this study. Blood samples were collected at 6, 24 and 3, 5 and 7 days after admission to determine plasma vitamin C, A, E, Uric acid concentration and plasma superoxide dismutase (SOD) and glutathione peroxidase (GPX) activity, and erythrocyte SOD activity. Blood levels of vitamins measured by chromatography, antioxidant enzyme activity was measured spectrophotometrically. The severity of the disease and changes using neurological score for evaluation. Thirty-seven healthy subjects, whose age and gender matched, were selected as the control group.