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目的:探讨噪声对胎鼠肺SP-B mRNA的影响。方法:Wistar孕鼠随机分为90dB组、75dB组和正常组,实验组孕鼠每日接受噪声刺激2h。孕7、14、19、21天采孕鼠血,RIA法测血清皮质酮水平;取孕19、21天胎鼠称重后取肺脏,RT-PCR法测胎肺SP-B mRNA表达水平。结果:随着噪声强度的增加、作用时间的延长,实验组孕鼠的血清皮质酮水平逐渐增加,胎鼠体重逐渐下降,胎肺SP-B mRNA表达水平逐渐下降,各组间比较差别有统计学意义。结论:大鼠孕期噪声暴露后,机体产生的皮质酮水平升高,可以导致胎鼠宫内发育迟缓(IUGR),胎肺发育不成熟,上述不良影响程度与噪声呈强度-效应、时间-效应关系,与机体产生的皮质酮呈浓度-效应关系。
Objective: To investigate the effect of noise on fetal SP-B mRNA in fetal rat. Methods: Pregnant Wistar rats were randomly divided into 90dB group, 75dB group and normal group. The pregnant rats in experimental group received daily noise stimulation for 2 hours. Pregnant rats were sacrificed on the 7th, 14th, 19th and 21st day of pregnancy, and serum levels of corticosterone were measured by RIA. Fetal rats were weighed and taken for lungs after 19 and 21 days of gestation. The expression of SP-B mRNA in fetal lung was measured by RT-PCR. Results: With the increase of noise intensity and prolongation of action time, the level of serum corticosterone in pregnant rats increased gradually, the body weight of fetal rats decreased gradually, and the expression level of SP-B mRNA in fetal lungs gradually decreased. There was statistical difference among the groups Significance of learning. CONCLUSIONS: After exposure to noise during pregnancy, elevated levels of cortisone produced by the body can lead to IUGR and immaturity of the fetal lungs. The degree of adverse effects and noise are intensity-effect, time-effect Relationship, and the body produces corticosterone concentration - effect relationship.