在生物学和医学研究中 ,细胞培养技术是最重要的手段之一 ,肿瘤研究中移植性肿瘤又称瘤株传代也是最基本的常用技术。关于瘤细胞培养后和瘤株建成后如何将细胞系和瘤株保存好是很值得研究的内容。人所共知冷冻保存瘤细胞和瘤株要比体内、体外传代保存更为优越 ,瘤细胞冻存后几 In biology and medical research, cell culture technology is one of the most important means of tumor research, transplant tumor, also known as tumor-passage is the most basic common technology. It is worthwhile to study how to preserve the cell lines and tumor strains after tumor cell culture and after the tumor is established. It is well known that the cryopreservation of tumor cells and tumor strains is more superior than in vivo and in vitro passage preservation, after the tumor cells are frozen