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目的 观察银杏黄酮对大鼠离体心脏缺血再灌注损伤的保护作用。方法 采用离体大鼠心脏Langendorff灌流 ,低灌缺血 40min ,再恢复常速灌流 30min ,造成心肌缺血再灌流损伤。利用该模型观察银杏黄酮对缺血再灌流心肌心缩力 (ST)、左室内压 (LVP)、心率 (HR)、冠脉流出量 (CBF)、冠脉流出液中乳酸脱氢酶 (LDH)、心肌丙二醛 (MAD)含量及超氧化物歧化酶 (SOD)活性的变化。结果 银杏黄酮 15mg/L和 30mg/L可改善缺血再灌注所致心肌收缩力及左室内压的降低 ,减少LDH释放量及心肌MAD生成量 ,并提高SOD活力。结论 银杏黄酮对离体心脏缺血再灌注有保护作用 ,其作用与直接清除自由基外 ,亦可能与SOD活性的维持有关。
Objective To observe the protective effect of flavonoids of Ginkgo biloba on myocardial ischemia/reperfusion injury in isolated rat hearts. Methods Langendorff perfusion was performed on isolated rat hearts, which was followed by 40 minutes of ischemia-reperfusion and 30 minutes of constant velocity perfusion, which caused myocardial ischemia and reperfusion injury. This model was used to observe the effects of Ginkgo biloba flavonoids on myocardial systolic force (ST), left ventricular pressure (LVP), heart rate (HR), coronary efflux (CBF), and lactate dehydrogenase (LDH) in coronary effluent. Changes in myocardial malondialdehyde (MAD) content and superoxide dismutase (SOD) activity. Results Ginkgo flavonoids 15 mg/L and 30 mg/L could improve myocardial contractility and left ventricular pressure induced by ischemia-reperfusion, reduce LDH release and myocardial MAD production, and increase SOD activity. Conclusion Ginkgo biloba flavone has a protective effect on isolated heart ischemia-reperfusion, and its action is related to the direct scavenging of free radicals, and may also be related to the maintenance of SOD activity.