论文部分内容阅读
目的探讨小核核糖核蛋白SmB及SmD抗原表位多肽在诊断系统性红斑狼疮(SLE)中的临床意义;寻找可能为抗原表位的短肽片段,建立简便快捷的酶联免疫吸附测定(ELISA)法,以提高SLE早期诊断水平。方法根据已知SmB及SmD蛋白的氨基酸序列,用不同蛋白质抗原表位图谱分析软件对其进行表位分析,经比对筛选后,化学合成SmB短肽序列2个(分别命名为B1及B2),和SmD短肽序列3个(分别命名D1、D2、D3),作为抗原对102例SLE患者和对照组[包括其他类型结缔组织病(CTD)153例和正常对照54名]进行血清相关抗体ELISA检测。结果经抗SmB抗原合成肽段B1和B2的ELISA检测结果显示,SLE中阳性率分别为32.4%和45.1%,特异度均达93.5%。而SmD抗原合成肽段D1、D2和D3的检测结果显示,其在SLE中阳性率分别为86.3%、72.6%和32.4%,特异度分别为61.4%、60.1%和60.1%,虽敏感度较高,但特异度均较差。结论SmB的B122-45和多聚脯氨酸区域B2193-204肽段对SLE诊断具有较高的抗原反应敏感度和特异度,这不仅为研究其蛋白功能结构及其在发病机制中的意义打下了基础,也为建立SLE诊断中新的指标和方法提供了思路。而SmD合成肽段ELISA检测的敏感度虽高,但特异度较差,表明其可能与天然蛋白的抗原表位结构及抗原反应性存在差异。
Objective To investigate the clinical significance of SmB and SmD epitope peptides in the diagnosis of systemic lupus erythematosus (SLE). To search short peptide fragments that may be epitopes and to establish a simple and rapid enzyme-linked immunosorbent assay (ELISA ) Method to improve the early diagnosis of SLE. Methods Based on the amino acid sequences of SmB and SmD proteins, epitopes were analyzed by different protein epitope mapping software. After screening, SmB short peptide sequences were chemically synthesized (B1 and B2 respectively) , And SmD short peptide sequence (named as D1, D2, D3 respectively) as antigens in 102 SLE patients and control group [including 153 cases of other types of connective tissue disease (CTD) and 54 normal controls] ELISA test. Results The results of ELISA for the synthesis of peptides B1 and B2 by anti-SmB antigen showed that the positive rates of SLE were 32.4% and 45.1% respectively, and the specificity was 93.5%. The results of the synthetic peptides D1, D2 and D3 of SmD antigen showed that the positive rates of Sif were 86.3%, 72.6% and 32.4% respectively, and the specificity was 61.4%, 60.1% and 60.1%, respectively High, but poor specificity. Conclusion The B122-45 and B2193-204 peptides of SmB have high antigen sensitivity and specificity for the diagnosis of SLE, which not only lay the foundation for studying the functional structure of the protein and its significance in pathogenesis The foundations also provide ideas for establishing new indicators and methods for SLE diagnosis. However, the sensitivity of the SmD synthetic peptide ELISA assay was high but its specificity was poor, indicating that it may be different from the epitope structure and antigen reactivity of the natural protein.