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目的评价红景天提取物对SD大鼠的母体及胎仔毒性。方法 SD孕鼠随机分为4组:红景天提取物3个剂量组(0.12、0.35、1.05g/kg)和阴性对照组,每组16只。各组均于受孕第7～16天(胚胎器官形成期)灌胃给予1.0mL/100(g·BW)的红景天提取物。受孕的0、7、12、16、20d称量体重,在妊娠的第20天,颈椎脱臼处死,计数活胎数、死胎数和吸收胎数;观察胎仔外观、称量其体重后,将每窝1/2的活胎仔乙醇固定、茜素红染色、甘油透明后检查骨骼发育情况。另1/2活胎仔经Bouins液固定后,检查内脏发育情况。结果在实验剂量范围内,红景天提取物各剂量组孕鼠的生殖能力、胚胎形成和胎仔外观、骨骼及内脏生长发育与阴性对照组相比差异均无统计学意义(均P>0.05)。结论该实验条件下未发现红景天提取物(0.12～1.05g/kg)对大鼠有胚胎毒性和致畸毒性。
Objective To evaluate the maternal and fetal toxicity of Rhodiola rosea extract to SD rats. Methods SD pregnant rats were randomly divided into 4 groups: Rhodiola extract 3 doses (0.12,0.35,1.05g / kg) and negative control group, 16 in each group. Each group was given intragastric administration of rhodiola extract 1.0 mL / 100 (g · BW) on the 7th to 16th day of pregnancy (embryogenesis period). Body weight was weighed at 0, 7, 12, 16 and 20 days of conception. On the 20th day of pregnancy, cervical dislocation was sacrificed and the number of live births, the number of stillbirths and the number of absorbed fetuses were counted. After observing the appearance of the fetuses and weighing their weight, Wound 1/2 live fetal ethanol fixed, alizarin red staining, check the bone growth after the transparent glycerol. Another 1/2 live fetus Bouins fluid fixed, check the visceral development. Results There were no significant differences in reproductive ability, embryogenesis and fetal appearance, skeletal and visceral growth and development of pregnant rats in each dose of Rhodiola rosea extract compared with the negative control group (all P> 0.05) . Conclusion Rhodiola rosea extract (0.12 ~ 1.05g / kg) was found not to cause embryo toxicity and teratogenicity in rats.