目的评价梅毒螺旋体重组抗原TpN17和TpN44.5在抗-TP-IgG检测中的应用价值。方法以上两种抗原包被聚苯乙烯板,用ELISA法测定188份临床诊断梅毒患者血清、38份类风湿性关节炎患者血清以及62份健康献血员血清中抗-TP-IgG,并与RPR及日本产TP-PA诊断试剂测定结果进行了比较。结果应用TpN17和TpN44.5对抗-TP-IgG的检测阳性率分别为98.9%和99.5%,与日本产TP-PA酶标试剂盒的符合率在98%以上。结论用重组TP抗原建立的ELISA检测血清中的抗-TP-IgG,特异性和敏感性均达到国际先进水平,是一种值得推广的检测抗-TP-IgG的常规方法。 Objective To evaluate the value of TpN17 and TpN44.5 in detection of anti-TP-IgG in Treponema pallidum. Methods Polystyrene plates were coated with the above two antigens. The serum levels of anti-TP-IgG in serum of 188 clinically diagnosed syphilis patients, 38 rheumatoid arthritis patients and 62 healthy donors were measured by ELISA. And Japanese TP-PA diagnostic reagents were measured results were compared. Results The positive rates of TpN17 and TpN44.5 for detection of anti-TP-IgG were 98.9% and 99.5%, respectively. The coincidence rates with TP-PA kit from Japan were above 98%. Conclusion The detection of anti-TP-IgG in serum by ELISA with recombinant TP antigen has reached the international advanced level. It is a worthy generalized method to detect anti-TP-IgG.