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目的建立幼猪油酸所致肺损伤模型,并观察最佳油酸成模剂量。方法取5～7周龄、体质量5～7 kg的健康雄性幼猪15只,随机分为实验组(n=9只)及对照组(n=6只)。各组动物均予经口气管插管后呼吸机机械通气,颈内动脉及颈外静脉置管。实验组幼猪予油酸80～150 mg.kg-1用9 g.L-1盐水稀释至15 mL,微量泵经其颈外静脉持续60 min缓慢注入右心室,对照组幼猪予9 g.L-1盐水15 mL微泵维持60 min静脉输入,取不同时间点(实验组0 h～成模时;对照组0～6 h)监测生命体征及各项呼吸力学、血气参数、血流动力学参数,至血氧分压/吸入氧体积分数[pa(O2)/FiO2]<39.4 kPa确认成模,实验组中3只动物立即处死,其余动物撤机后维持存活1周,实验结束后取左侧肺组织行病理学检测。结果油酸泵入3～14 h,实验组pa(O2)/FiO2值出现显著下降,与对照组比较差异有统计学意义(P<0.05),肺形态学达急性肺损伤标准。其中实验组7只幼猪至3～8 h pa(O2)/FiO2值均明显下降,油酸剂量为80～120 mg.kg-1,另2只成模时间分别为11 h及14 h,使用油酸剂量达150 mg.kg-1。对照组至通气6 hpa(O2)/FiO2值在各时间点差异均无统计学意义。实验组动物在通气起始,体循环动脉血压(SBP)及心率较为稳定,随着通气时间延长,实验组及对照组SBP、心率变化无统计学意义。结论使用油酸80～150 mg.kg-1可成功建立幼猪油酸所致肺损伤模型,最佳油酸成模剂量为80～120 mg.kg-1。
Objective To establish a model of lung injury induced by oleic acid in young pigs and observe the best dosage of oleic acid. Methods Fifteen healthy male piglets (5-7 weeks old and 5-7 kg body weight) were randomly divided into experimental group (n = 9) and control group (n = 6). All animals were ventilated by mechanical ventilation of the ventilator after oral intubation, internal carotid artery and external jugular vein catheterization. The experimental group was fed with oleic acid 80-150 mg.kg-1 diluted to 15 mL with 9 gL-1 saline, and the micro-pump was slowly infused into the right ventricle via the external jugular vein for 60 min. The control group was given 9 gL-1 Saline 15 mL micropumps were maintained for 60 min. The vital signs, respiratory mechanics, blood gas parameters and hemodynamic parameters were monitored at different time points (experimental group 0 h ~ modeling time; control group 0 ~ 6 h) To the oxygen partial pressure / inhaled oxygen volume fraction [Pa (O2) / FiO2] <39.4 kPa confirmed that the model, the experimental group of three animals were sacrificed immediately, the remaining animals to maintain survival for 1 week after weaning, left after the experiment Pathological examination of lung tissue. Results After oleic acid was pumped for 3-14 h, the pa (O2) / FiO2 value of the experimental group decreased significantly compared with that of the control group (P <0.05). The lung morphology reached the standard of acute lung injury. The experimental group of 7 young pigs to 3-8 hPa (O2) / FiO2 values were significantly decreased, oleic acid dose of 80 ~ 120 mg.kg-1, and the other two molding time was 11 h and 14 h, respectively, The use of oleic acid dose of 150 mg.kg-1. Control group to ventilation 6 hpa (O2) / FiO2 values at any time point difference was not statistically significant. The experimental group had stable SBP and heart rate at the beginning of ventilation, and there was no significant difference in SBP and heart rate between the experimental group and the control group as the ventilation time prolonged. Conclusion Oleic acid-induced lung injury model was successfully established using 80-150 mg · kg-1 oleic acid. The optimum dosage of oleic acid was 80-120 mg · kg-1.