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研究了进口保健食品中褪黑激素的提取、净化、定性、定量的高效液相色谱(HPLC)法条件,建立了HPLC法快速测定保健食品中褪黑激素的可靠方法。本方法具有操作简便、快速、结果准确可靠、再现性好的特点。褪黑激素在两种进口保健食品麦乐通中三个水平的添加回收率在97.6％～105.7％之间,变异系数(n=8)在0.27％～1.64％之间,该方法在0.01mg/ml～0.2mg/ml浓度范围内有良好的线性关系,相关系数r=0.9998;最低检出浓度1.1μg/ml;最小检出量2.8ng。
The extraction, purification, qualitative and quantitative conditions of melatonin in imported health food were studied, and a reliable method for rapid determination of melatonin in health food by HPLC was established. The method has the advantages of simple and quick operation, accurate and reliable result and good reproducibility. The recoveries of melatonin at three levels of the two imported health foods, Melengtong, ranged from 97.6% to 105.7%, and the coefficients of variation (n = 8) ranged from 0.27% to 1.64% / ml ~ 0.2mg / ml concentration range has a good linear relationship, the correlation coefficient r = 0.9998; minimum detectable concentration of 1.1μg / ml; the minimum detectable amount of 2.8ng.