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目的分析中国特发性无精子症和少精子症患者雄激素受体(AR)基因(CAG)n微卫星多态性并探讨该多态性与精子生成障碍发生的关系。方法应用PCR和变性聚丙烯酰胺凝胶电泳分析技术对52例少精子症患者和31例无精子症患者的外周血标本进行CAG重复数测定,分析该微卫星多态性和精子生成障碍发生的关系。结果少精子症患者组和无精子症患者组CAG重复数均数分别为22.19和22.13,CAG重复数≥28的百分率分别为1.9%和3.2%,比例逐渐升高。结论AR基因(CAG)n微卫星的CAG重复数在中国男性不育患者中呈现多态性,与精子生成障碍发生的关系有待进一步研究。
Objective To analyze the microsatellite polymorphism of androgen receptor (AR) gene (CAG) n in Chinese patients with idiopathic azoospermia and oligospermia and to explore the relationship between the polymorphism and the occurrence of spermatogenesis. Methods PCR and denaturing polyacrylamide gel electrophoresis analysis of 52 cases of oligospermia patients and 31 cases of azoospermic patients with CAG repeat determination of the number of samples to analyze the microsatellite polymorphisms and spermatogenesis disorders relationship. Results The average numbers of CAG repeat in patients with oligospermia and azoospermia were respectively 22.19 and 22.13, and the percentages of CAG repeat ≥ 28 were 1.9% and 3.2%, respectively, with the proportion increasing gradually. Conclusion CAG repeat of AR gene (CAG) n microsatellite shows polymorphism in male infertility patients in China, and its relationship with spermatogenesis disorder remains to be further studied.