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应用单克隆抗体免疫荧光技术(MIF)检测了青枯病在马铃薯种薯上的潜伏侵染。测试结果表明,MIF检测青枯病茵的灵敏度为2×10~3～2×10~4细菌/毫升。787.5倍荧光显微镜,每视野平坶有5个以上典型荧光细菌,即可判别为带菌(阳性),而1～4个荧光细菌则视为可疑,需作生物测定。从种薯脐部取样,经组织匀浆后采用低、高速离心收集细菌体,可提高样品青枯菌的检出率。
Monoclonal antibody immunofluorescence (MIF) was used to detect the latent infection of bacterial wilt on potato seedlings. The test results show that the sensitivity of MIF detection of bacterial wilt is 2 × 10 ~ 3 ~ 2 × 10 ~ 4 bacteria / ml. 787.5 times the fluorescence microscope, each flat field with more than 5 typical fluorescent bacteria can be identified as carrying (positive), and 1 to 4 fluorescent bacteria are considered suspicious to be biomedical. Samples from the umbilical tubercle, after homogenization using low-speed, high-speed centrifugation to collect bacteria, can increase the detection rate of R. solanacearum samples.