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目的:研究2型糖尿病对外周动脉疾病患者血浆内的血管内皮生长因子(VEGF-A)及其水溶性受体(s VEGFR-1和s VEGFR-2)浓度的影响。创新点:首次研究了2型糖尿病对外周动脉疾病患者血浆内s VEGFR-1和s VEGFR-2浓度的影响。方法:选取46个外周动脉疾病患者,根据有无2型糖尿病分为糖尿病组(15例)和无糖尿病组(31例),另选30个健康志愿者为正常对照组。采用酶联免疫吸附法(ELISA)检测他们血浆中VEGF-A及s VEGFR-1和s VEGFR-2的浓度,然后通过对比各组浓度研究2型糖尿病的影响。结论:与正常对照组相比,外周动脉疾病患者具有较高的VEGF-A浓度(2型糖尿病组P=0.000007,非糖尿病组P=0.0000001)以及较低的s VEGFR-2浓度(2型糖尿病组P=0.02,非糖尿病组P=0.00001)。同时,2型糖尿病组比非糖尿病组具有较低的VEGF-A浓度及较高的s VEGFR-1和s VEGFR-2浓度。研究结果表明:无论2型糖尿病是否共存,缺氧是导致血管生成的一个关键的刺激因素;同时,高血糖状态对下肢的血管生成有抑制作用。
Objective: To investigate the effect of type 2 diabetes on the plasma concentrations of vascular endothelial growth factor (VEGF-A) and its soluble receptors (s VEGFR-1 and s VEGFR-2) in patients with peripheral arterial disease. Innovative point: The first study of type 2 diabetes in patients with peripheral arterial disease plasma s VEGFR-1 and s VEGFR-2 concentration. Methods: Forty-six patients with peripheral arterial disease were divided into diabetic group (n = 15) and non-diabetic group (n = 31) according to the presence or absence of type 2 diabetes. Another 30 healthy volunteers were selected as normal control group. The concentrations of VEGF-A and VEGFR-1 and VEGFR-2 in their plasma were detected by enzyme-linked immunosorbent assay (ELISA), and then the effect of type 2 diabetes was studied by comparing the concentration of each group. CONCLUSIONS: Patients with peripheral arterial disease had higher concentrations of VEGF-A (type 2 diabetes mellitus 0.000007, non-diabetic mellitus 0.0000001) and lower concentrations of s VEGFR-2 than type 2 diabetes mellitus (type 2 diabetes mellitus Group P = 0.02, non-diabetic group P = 0.00001). In the meantime, type 2 diabetes patients had lower concentrations of VEGF-A and higher concentrations of s VEGFR-1 and s VEGFR-2 than non-diabetic patients. The results show that: whether type 2 diabetes coexists, hypoxia is a key stimulus for angiogenesis; at the same time, hyperglycemia can inhibit angiogenesis in the lower extremities.