旋毛虫病免疫诊断的可靠性主要取决于抗原的质量和特异性。假阳性是由于所用的粗抗原未经纯化。作者将大鼠肌肉消化分离的新鲜幼虫经PBS洗涤后1000g离心10min。沉淀物经冻融后置入含10mmol/L的Tris-HCl(pH8,2mmol/L EDTA)、抑胃酶素(1μg/ml)、亮肽素(1μg/ml)和2mmol/LPMSF的缓冲液中制成匀浆,不溶部分经离心沉淀后重复浸出共4次。最后将上清与残渣混匀30min后再50000g离心1h,上清 The reliability of diagnosis of Trichinellosis depends mainly on the quality and specificity of the antigen. False positive is due to the crude antigen used without purification. The authors digested muscle fresh rat larvae were washed with PBS 1000g 10min. After the precipitate was thawed and thawed, a buffer solution containing 10 mmol / L of Tris-HCl (pH 8, 2 mmol / L EDTA), pepstatin (1 g / ml), leupeptin (1 g / ml) and 2 mmol / Made of homogenate, insoluble part of the precipitate after centrifugation repeated leaching a total of 4 times. Finally, the supernatant and the residue mix 30min and then centrifuged 50000g 1h, supernatant