论文部分内容阅读
以不同剂量的紫外线 (UV)照射谷子 (Se tariaitalicacv .Shanxi)原生质体为供体与普通小麦 (Triticumaestivum)济南 1 77和 99P的原生质体用PEG法诱导融合。利用同工酶 ,RAPD和染色体分析再生的克隆及白化苗。从小麦济南 1 77与谷子融合再生的 86个克隆中 ,2 4个被鉴定为杂种克隆 ;而小麦 99P与谷子融合再生的 6 7个克隆中 ,2 7个被鉴定为杂种克隆。虽然用作融合材料的双亲培养细胞均丧失再生能力 ,但是部分来源于低剂量UV处理的杂种克隆再生了绿点、根和白化苗。证明小麦体细胞杂交中的双亲再生能力互补现象也存在于远缘族间融合组合中。讨论了小麦远缘族间融合未能再生绿色植株的原因
The fusion of protoplasts of Sertiaitalicacv.Shanxi protoplasts and Triticum aestivum Jinan 1 77 and 99P was induced by different doses of UV radiation. Regeneration of clones and albino seedlings using isozymes, RAPD and chromosome analysis. Of the 86 clones regenerated from the wheat Jinan 77 with millet, 24 were identified as hybrid clones; of the 67 clones regenerated from wheat 99P fused with millet, 27 were identified as hybrid clones. Although both parents cultured cells used as a fusion material lost their ability to regenerate, hybrid clones partially derived from low-dose UV treatment regenerated green spots, roots and albino seedlings. It is proved that complementarity of parents’ reproducibility in somatic hybridization of wheat is also present in the inter-distant-family fusion combination. This paper discusses the reason why the wheat distant inter-family fusion fails to regenerate green plants