论文部分内容阅读
为探讨PCR在小儿支原体肺炎诊断中的实际应用价值，对47例临床疑诊支原体肺炎的病儿做了咽拭子PCR－MP测定，同时做了血冷凝集试验（CAT）。结果：PCR－MP阳性21例，真阳性15例，假阴性工例，敏感度为938％。假阳性6例，占检测标本的12．8％，占检测报告阳性总数的28．6％，CAT阳性12例。确诊支原体肺炎16例，其中15例经PCR早期确诊。结论：PCR－MP测定有利于支原体肺炎的早期诊断，但存在假阳性及假阴性。结合临床特点应用PCR－MP将有助于提高支原体肺炎早期确诊率。
In order to explore the practical value of PCR in the diagnosis of pediatric mycoplasma pneumonia, throat swab PCR-MP assay was performed in 47 cases of suspected mycoplasmal pneumonia in children with clinical isolates, and blood coagulation test (CAT) was also performed. Results: 21 cases were positive by PCR-MP, 15 cases were true positive and false negative cases were 938%. False positive in 6 cases, accounting for 12.8% of the test specimens, accounting for 28.6% of the total positive test report, CAT positive in 12 cases. Mycoplasma pneumonia was diagnosed in 16 cases, of which 15 cases were diagnosed early by PCR. Conclusion: PCR-MP assay is good for mycoplasma pneumonia early diagnosis, but there are false positives and false negatives. Combined with clinical features of PCR-MP will help to improve the early diagnosis of mycoplasma pneumonia.