目的 :建立HPLC法测定化痰止咳片中黄芩苷的含量。方法 :采用C18化学键合硅胶柱 ,以甲醇 水 醋酸 (43∶5 7∶1)为流动相 ,UV检测波长 2 76nm。结果 :平均回收率为 98.3% ,RSD为 0 .5 % (n =5 ) ,黄芩苷进样量与吸收峰积分值呈良好的线性关系。线性范围 0 .2 5～ 2 .5 μg。 结论 :本法测定化痰止咳片中黄芩苷的含量 ,结果准确 ,重复性好 Objective : To establish a HPLC method for the determination of baicalin in Huatan Zhike Tablets. Method : C18 chemically bonded silica gel column was used. The mobile phase was methanol acetic acid (43∶5 7∶1) and UV detection wavelength was 2 76nm. Results: The average recovery was 98.3%, the RSD was 0.5% (n =5), and there was a good linear relationship between the injection amount of baicalin and the integral value of the absorption peak. The linear range is 0.25 to 2.5 μg. Conclusion: This method was used to determine the content of baicalin in Huayu Zhike Tablets. The results were accurate and the repeatability was good.