目的 :以HPLC梯度洗脱测定三七中三七皂苷R1含量。方法 :采用SphericorbNH2 柱 ,流动相为CH3CN CH3OH H2 O ,梯度洗脱浓度 5 5∶30∶15→ 70∶2 0∶10 ,波长 2 10nm ,外标一点法测定。结果 :以峰面积计算 ,三七皂苷R1在 5～ 40 μg·ml-1呈线性相关 ,γ =0 .994,最低检测限为 0 .6 μg·ml-1(S/N =3)。平均回收率 10 2 .6 % ,RSD为 1.43 % .日内误差RSD =1.7% (n =5 ) ,日间误差RSD =3 .0 % (n =4)。结论 :本法专属性强 ,结果准确 ,操作简捷 Objective : To determine the content of notoginsenoside R1 in Panax Notoginseng by HPLC gradient elution. METHODS: The mobile phase was CH3CN CH3OH H2O using a Sphericorb NH2 column. The gradient elution was 5:30:15:1→70:2 0:10, the wave length was 210 nm. RESULTS: Based on the peak area, notoginsenoside R1 was linearly correlated at 5-40 μg·ml-1 with γ=0.994, and the detection limit was 0.6 μg·ml-1 (S/N = 3). The average recovery rate was 102.6% and the RSD was 1.43%. The intraday error was RSD = 1.7% (n = 5), and the daytime error was RSD = 3.0% (n = 4). Conclusion: This law is unique, accurate, and simple to operate.