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介绍了在利用光学成像现场测量系统对海洋微生物进行分类研究的领域,通过几十年的不懈努力所取得的技术进展及其背后的科学原理。其中一项是进行了数十年的研究工作,主要是利用面激光作为光源照明海水从而观察荧光粒子的分布情况。通过将其安装在一个自由沉降的自主航行器上,观察到了以往通过体叶绿素荧光测量法无法观测的荧光粒子分层所形成的“神秘峰值”。这些荧光粒子层很可能是大型浮游草食生物的食物,是海洋上层生态环境的重要特征。最近Jaffe博士的团队研发了一种水下成像光谱显微镜,利用该设备对通过端口的生物进行成像探测,可以在不改变水体环境的前提下对不同种类的大型、微型浮游生物进行识别。
The field of classification of marine microorganisms using optical imaging field measurement systems is introduced. The technological advances and the scientific principles behind them have been introduced through decades of unremitting efforts. One of them is a research work carried out for decades, mainly using surface laser as a light source to illuminate the seawater to observe the distribution of fluorescent particles. By installing it on a free-fall autonomy aircraft, a “mysterious peak” of the stratification of fluorescent particles that was previously unobserved by bulk chlorophyll fluorescence was observed. These layers of florescent particles are likely to be food for large planktonic herbivores and are an important feature of the marine upper ecosystem. Recently Dr. Jaffe’s team developed an underwater imaging spectroscopy microscope that allows imaging of creatures that pass through ports to identify different types of large, miniature plankton without changing the water environment.