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目的探讨低剂量辐射对小鼠移植肿瘤细胞的凋亡、细胞周期以及Bcl-2的影响。方法昆明种雄性小鼠左后肢腹股沟皮下接种S180肉瘤细胞,接种后7天γ射线全身照射75mGy,照射后24、48小时分别处死直接测量肿瘤大小变化,取肿瘤组织分别进行流式细胞仪分析凋亡、细胞周期以及免疫组化染色半定量分析凋亡相关蛋白Bcl-2表达的变化。结果与直接荷瘤组相比,低剂量照射组肿瘤生长缓慢(P<0.05),24小时后肿瘤细胞阻滞于G1期,Bcl-2蛋白表达下降,48小时后肿瘤细胞凋亡增加(P<0.001)。结论低剂量辐射可使机体肿瘤细胞阻滞于G1期并通过凋亡相关蛋白表达变化导致肿瘤细胞凋亡增加,明显提高机体抗肿瘤的作用,具有肿瘤治疗和辅助放化疗的实际临床意义。
Objective To investigate the effects of low dose radiation on apoptosis, cell cycle and Bcl-2 in tumor-bearing mice. Methods Male Kunming mice were inoculated subcutaneously into the inguinal region of the left hindlimb with S180 sarcoma cells. Whole body irradiation with γ-rays was performed at 75 mGy 7 days after inoculation. Tumor size was measured directly at 24 and 48 hours after irradiation. Tumor tissues were analyzed by flow cytometry Cell cycle and immunohistochemical staining were used to detect the expression of Bcl-2. Results Compared with the direct tumor-bearing group, the tumor growth was slow in the low-dose irradiation group (P <0.05). After 24 hours, the tumor cells arrested in the G1 phase and the expression of Bcl-2 protein decreased. After 48 hours, the tumor cell apoptosis increased <0.001). Conclusion Low-dose radiation can block tumor cells in G1 phase and increase the apoptosis of tumor cells through the expression of apoptosis-related proteins. It can significantly improve the anti-tumor effect of the tumor and have the clinical significance of tumor treatment and adjuvant chemoradiotherapy.