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目的研究儿童发生呼吸道感染与鼻病毒的相关性,为防治鼻病毒感染提供重要的预防和治疗依据。方法选取2014年12月-2016年12月医院发生呼吸道感染的患儿226例,并使用RT-PCR对患儿进行人鼻病毒(HRV-A、HRV-B、HRV-C)等检测,并对产生阳性反应的标本行进化树分析、VP4/VP2衣壳蛋白质编码区基因和序列片段进行测定。结果在226例患者中,有44例患儿HRV检出阳性,阳性率19.47%,且性别间阳性率差异无统计学意义;普通感冒患儿的HRV阳性率为20.78%,支气管炎为17.24%,肺炎为19.83%,支气管哮喘未检测出阳性,不同疾病类型的阳性率差异均无统计学意义;HRV不同基因型与相同基因型的各核苷酸序列的同源性分别为55.5%~65.8%。结论 HRV是引起医院儿童急性呼吸道感染的重要因素,鼻病毒阳性患儿临床病症与呼吸道感染病情严重程度密切相关。
Objective To study the correlation between respiratory infection and rhinovirus in children and to provide important prevention and treatment basis for the prevention and treatment of rhinovirus infection. Methods Totally 226 children with respiratory tract infection in hospital from December 2014 to December 2016 were selected and detected by RT-PCR for human rhinovirus (HRV-A, HRV-B, HRV-C) Phylogenetic tree analysis of positive reaction samples and determination of VP4 / VP2 capsid protein coding region genes and sequence fragments. Results Of the 226 patients, HRV positive was detected in 44 children (19.47%), and there was no significant difference in the positive rates of gender between the sexes. The prevalence of HRV in children with common cold was 20.78% and bronchitis was 17.24% , Pneumonia was 19.83%, bronchial asthma was not detected positive, the positive rate of different disease types were no significant difference; HRV different genotypes and the same genotype nucleotide sequence homology was 55.5% ~ 65.8 %. Conclusion HRV is an important factor in causing acute respiratory infection in hospital children. The clinical symptoms of rhinovirus positive children are closely related to the severity of respiratory tract infection.