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目的:探讨夏枯草醇提取物对胸主动脉环的舒张作用及其作用机制。方法:采用大鼠离体胸主动脉环灌流模型法,观察夏枯草醇提取物(0.05,0.10和0.15mg.ml-1)对苯肾上腺素(PE)1.0μmol.L-1和KCl 50 mmol.L-1预收缩的胸主动脉环张力的影响。结果:夏枯草醇提取物对离体大鼠内皮完整和去内皮的胸主动脉环均有浓度依赖性的舒张作用,而对苯肾上腺素(PE)预收缩血管的舒张作用是内皮依赖性的。用一氧化氮合酶抑制L-NAME和岛苷酸环化酶抑制剂MB预处理后,两者的血管舒张作用均被阻断。但用环氧合酶抑制剂吲哚美辛,不能阻断夏枯草醇提取物引起的舒张血管作用。结论:夏枯草醇提取物可能是通过NO-鸟苷酸环化酶途径产生内皮依赖性的血管舒张作用。
Objective: To investigate the relaxation effect of Prunella vulgaris extract on the thoracic aortic rings and its mechanism. Methods: Rat model of isolated thoracic aorta was used to observe the effects of Prunella vulgaris extract (0.05, 0.10 and 0.15 mg.ml-1) on the expression of phenylephrine (PE) 1.0 μmol·L-1 and KCl 50 mmol Effect of L-1 precontracted thoracic aortic ring tension. RESULTS: Prunella vulgaris extract had a concentration-dependent relaxation effect on the intact endothelium and endothelium of the thoracic aorta in vitro, whereas the relaxation of prerendered phenylephrine (PE) was endothelium-dependent . Nitric oxide synthase inhibition of L-NAME and the island glycosidase inhibitor MB pretreatment, both vasodilatation were blocked. However, the cyclooxygenase inhibitor indomethacin did not block the vasodilator action induced by the Prunella extract. CONCLUSION: Prunella vulgaris extract may be an endothelium-dependent vasodilator via the NO-guanylate cyclase pathway.