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目的对2012年深圳市龙岗区某酒楼发生的一起食物中毒事件的病原体进行分离鉴定和同源性分析。方法对2012年深圳市龙岗区某酒楼发生的食物中毒事件进行现场调查并采集患者肛拭子15份、厨工肛拭子3份和手拭子1份、可疑食品1份以及物表涂抹拭子2份。通过分离鉴定、生化试验和血清学分型确定菌型,再使用脉冲场电泳(PFGE)分型方法对同期患者中相同菌型的菌株进行同源性分析。食物中毒菌株使用K-B进行药敏分析。结果流行病学调查及实验室病原学检测证实本次事件是一起由肠炎沙门菌引起的食物中毒。共分离到16株肠炎沙门菌,其中患者、厨工及可疑食品分离出的肠炎沙门菌同源性达到95%以上,与同一时期散发临床患者分离的肠炎沙门菌同源性为89.4%,可判断为来自同一克隆株。结论此次事件是一起由肠炎沙门菌引起的食物中毒,分子流行病学的证据显示其可能的传播途径为厨工带菌者污染食品烧鸡,被患者进食后引起感染。
OBJECTIVE To isolate and identify the pathogens of a food poisoning incident occurred in a restaurant in Longgang District, Shenzhen in 2012. Methods On-site investigation of food poisoning incidents occurred in a restaurant in Longgang District in 2012 and collected 15 samples of patients’ anal swabs, 3 cooker swabs, 1 swab, 1 suspicious food and swab 2 copies. The isolates were determined by isolation and identification, biochemical tests and serotyping, and the PFGE typing was used to analyze the homology of strains with the same bacterial type in the same period. Food poisoning strains using K-B for susceptibility analysis. Results Epidemiological investigations and laboratory etiological tests confirmed that this incident was caused by food poisoning caused by Salmonella enteritidis. A total of 16 Salmonella enteritidis isolates were isolated, of which Salmonella enteritidis isolates from patients, chefs and suspicious foods were more than 95% homologous to Salmonella enteritidis isolated from clinical patients in the same period, accounting for 89.4% It is judged that it is from the same clone. Conclusion The incident was caused by food poisoning caused by Salmonella enteritidis. Molecular epidemiological evidence shows that the possible route of transmission is that kitchen workers carry contaminated food, which causes infection after eating.