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通过大肠杆菌（Ｅ．ｃｏｌｉ）核苷磷酸化酶的转脱氧核糖基作用，从胸腺嘧啶和２＇－脱氧核苷合成胸苷。优化了以ｄＵ为底物合成胸苷的反应条件。以３０ｍｍｏｌ／Ｌ ｄＵ为底物其合成胸苷的转化率可达５５．３％；以ｄＡ、ｄＣ、ｄＵ、ｄＧ的混合物为底物转化率为５２．６％；以ＤＮＡ降解后的２＇－脱氧核苷混合液作底物，反应液中胸苷浓度从９．２ｍｍｏｌ／Ｌ提高到１７．９ｍｍｏｌ／Ｌ。
Thymidine is synthesized from thymine and 2’-deoxynucleosides by deoxyribosylation of E. coli nucleoside phosphorylase. The reaction conditions for the synthesis of thymidine using dU as a substrate were optimized. The conversion rate of synthetic thymidine was 55.3% with 30 mmol / L dU as substrate. The conversion of dA, dC, dU and dG was 52.6% - deoxynucleoside mixture as a substrate, thymidine concentration in the reaction solution increased from 9.2mmol / L to 17.9mmol / L.