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目的 初步筛选肿瘤血管内皮标志物8(tumorendothelialmarker 8,TEM 8)HLA A2 1限制性低亲和性CTL表位,并预测修饰后的表位与HLA A2 1之间亲和性的变化。方法 采用超基序、3D QSAR、蛋白酶解预测等相结合的办法筛选HLA A2 1限制性低亲和性CTL表位,并通过氨基酸置换适当修饰,最后对部分候选的多肽进行分子动力学模拟。结果 筛选出8个低亲和性CTL候选表位,经修饰后的表位与HLA A2 1之间的亲和性均有不同程度的提高。结论 初步预测和修饰的表位系列可为下一步实验提供了依据。
OBJECTIVE: To screen for HLA A2 1-restricted low-affinity CTL epitopes in tumorendothelial marker 8 (TEM 8) and to predict the change of affinity between the modified epitope and HLA A2 1. Methods HLA A2 1 restricted low affinity CTL epitopes were screened by combining super motifs, 3D QSARs and proteolytic prediction. The epitopes were modified by amino acid substitution. Finally, some candidate peptides were subjected to molecular dynamics simulation. Results Eight low affinity CTL candidate epitopes were screened, and the affinity between the modified epitope and HLA A2 1 was increased to some extent. Conclusion Preliminary prediction and modification of the epitope series may provide the basis for the next experiment.