论文部分内容阅读
目的:研究癌组织P16蛋白及mRNA的表达情况。方法:将90例行喉癌切除术的患者作为研究对象,收集其术中切出的肿瘤组织和正常组织,采用免疫组织化学的方法检查其组织内P16蛋白的表达水平,并用real-time PCR检查其P16mRNA的表达水平。结果:喉癌组织中P16基因mRNA含量、免疫组化阳性例数均小于正常组织。在肿瘤组织中mRNA水平和免疫组化阳性例数呈现TNMⅠ期>Ⅱ期>Ⅲ期>Ⅳ期的特点。结论:与正常组织相比,喉癌组织中P16蛋白及mRNA的表达会发生明显的减少。
Objective: To study the expression of P16 protein and mRNA in cancer tissue. Methods: Totally 90 patients with laryngectomy were included in this study. Tumor tissues and normal tissues were collected. Immunohistochemistry was used to examine the expression of P16 protein in the tissues. Real-time PCR Check its P16 mRNA expression levels. Results: The mRNA and protein expression of P16 in laryngeal squamous cell carcinoma tissues were lower than those in normal tissues. The level of mRNA and the number of immunohistochemical positive cases in TNM stage showed the characteristics of TNM stage> stage> stage> stage> IV. Conclusion: Compared with normal tissues, the expression of P16 protein and mRNA in laryngeal cancer tissues will be significantly reduced.