为了鉴定表达恒河猴CD40L胞外段基因的重组腺病毒载体在恒河猴未成熟树突状细胞(immature dendritic cell,imDC)中的稳定表达。应用磁珠法分选健康恒河猴骨髓血中CD34+阳性细胞,IL-4及GM-CSF诱导分化为imDC,并通过流式细胞术鉴定纯度及成熟度。使用HEK293细胞包装腺病毒载体pAd-CMV-sCD40L,并感染恒河猴imDC。采用PCR以及Western Blot技术鉴定CD40L在imDC中的表达。结果显示恒河猴CD40L胞外段基因可以在恒河猴imDC中稳定的表达。 In order to identify the stable expression of recombinant adenovirus vector expressing the CD40L extracellular domain gene of rhesus monkey in immature dendritic cells (imDC). The CD34 + positive cells, IL-4 and GM-CSF in bone marrow of healthy rhesus macaques were sorted into imDC by magnetic beads method, and the purity and maturity were identified by flow cytometry. The adenovirus vector pAd-CMV-sCD40L was packaged using HEK293 cells and infected with rhesus imDC. The expression of CD40L in imDC was identified by PCR and Western Blot. The results show that the rhesus CD40L extracellular domain gene can be stably expressed in rhesus monkey imDC.