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目的:探讨阿托伐他汀钙对血管紧张素(Ang)Ⅱ诱导的高血压患者肠系膜动脉平滑肌细胞内NADPH氧化酶-活性氧通路传导和促细胞增殖的干预作用。方法:用培养的第2至4代高血压患者肠系膜动脉平滑肌细胞作为实验标本;以四甲基偶氮唑盐(MTT)法比色测定各组平滑肌细胞增殖率;流式细胞仪检测被双氢乙酰乙酸-二氯荧光黄(DCFH-DA)标记的细胞内活性氧;逆转录聚合酶链式反应(RT-PCR)方法检测细胞内p22phox蛋白mRNA的表达变化。结果:与空白对照组比较,AngⅡ组的细胞增殖率、细胞内活性氧生成量、细胞内p22phoxmRNA的表达均明显增高。与浓度为10-6mol/LAngⅡ孵育组比较,AngⅡ加阿托伐他汀钙组的细胞增殖率、细胞内活性氧、细胞内p22phox蛋白mRNA的表达均明显下降。结论:阿托伐他汀钙可以部分抑制高血压患者体内AngⅡ的促平滑肌细胞增殖及诱导细胞内NADPH氧化酶-活性氧通路。
AIM: To investigate the effects of atorvastatin calcium on NADPH oxidase - ROS pathway and cell proliferation in mesenteric artery smooth muscle cells induced by angiotensin Ⅱ (Ang Ⅱ). Methods: The mesenteric artery smooth muscle cells from the second to the fourth generation of hypertensive patients were used as experimental specimens. The proliferation of smooth muscle cells in each group was determined by MTT colorimetric assay. Flow cytometry The intracellular reactive oxygen species (ROS) labeled with DCFH-DA and the expression of p22phox mRNA were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Results: Compared with the blank control group, the cell proliferation rate, intracellular reactive oxygen species production and intracellular p22phox mRNA expression in AngⅡ group were significantly increased. Compared with the 10-6mol / LAngⅡgroup, the cell proliferation rate, intracellular reactive oxygen species and intracellular p22phox mRNA expression in AngⅡ plus atorvastatin calcium group were significantly decreased. Conclusions: Atorvastatin calcium can partially inhibit Ang Ⅱ-induced smooth muscle cell proliferation and induce intracellular NADPH oxidase-reactive oxygen species (ROS) pathways in hypertensive patients.