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目的探讨代谢综合征(MS)大鼠心脏血管紧张素转换酶2(ACE2)的表达及甘肃黄芪对其影响。方法 60只雄性Wistar大鼠随机分为6组,每组10只,分别为正常对照组、果糖诱导的MS模型组、MS+缬沙坦20mg/(kg.d)组(缬沙坦组)、MS+黄芪1.5g/(kg.d)组(低剂量组)、MS+黄芪3.0g/(kg.d)组(中剂量组)、MS+黄芪6.0g/(kg.d)组(高剂量组)。采用果糖诱导的代谢综合征大鼠模型:正常对照组饮用自来水,其余各组饮用30%果糖水代替自来水,并同时给予相应的药物灌胃,持续6周。6周末,用放射免疫法检测心脏血管紧张素Ⅱ(AngⅡ);用实时定量RT-PCR和免疫蛋白印迹法检测各组大鼠心脏ACE2/ACE的表达。结果缬沙坦组大鼠心脏AngⅡ浓度较MS组增高[(77.1±2.6)比(59.8±2.6)ng/g,P<0.01],黄芪低、中、高剂量组心脏AngⅡ浓度均较MS组降低[分别为(55.3±5.2),(51.4±2.6),(45.6±2.7)比(59.8±2.6)ng/g,均P<0.05]。与正常对照组相比,MS模型组大鼠ACEmRNA和蛋白表达上调,ACE2mRNA和蛋白表达下调。缬沙坦和黄芪能提高MS大鼠ACE2mRNA和蛋白表达,降低ACEmRNA和蛋白表达[ACE2蛋白:缬沙坦组(1.47±0.22),黄芪低剂量组(1.12±0.31),中剂量组(1.12±0.19),高剂量组(1.49±0.20)比MS组(0.71±0.10),均P<0.05;ACE蛋白:缬沙坦组(1.06±0.21),黄芪低剂量组(1.75±0.21),中剂量组(1.43±0.22),高剂量组(1.15±0.20)比MS组(2.09±0.22),均P<0.05]。结论 MS大鼠心脏局部ACE2表达降低,ACE表达升高,ACE2和ACE水平失衡;甘肃黄芪可以提高ACE2的表达,降低ACE表达,改善心脏局部ACE2/ACE失衡。
Objective To investigate the expression of cardiac angiotensin converting enzyme 2 (ACE2) in rats with metabolic syndrome (MS) and the effect of Gansu Astragalus membranaceus on it. Methods Sixty male Wistar rats were randomly divided into 6 groups (n = 10): normal control group, model group induced by fructose, MS + valsartan 20mg / (kg.d) group (valsartan group) MS + astragalus 1.5g / (kg.d) group (low dose group), MS + Astragalus 3.0g / (kg.d) group (middle dose group), MS + astragalus 6.0g / . Rat model of metabolic syndrome induced by fructose: normal control group drinking tap water, the remaining groups drinking 30% fructose water instead of tap water, while giving the appropriate drug gavage for 6 weeks. Cardiac angiotensin Ⅱ (AngⅡ) was detected by radioimmunoassay at the end of 6th week. The expression of ACE2 / ACE in heart of each group was detected by real-time quantitative RT-PCR and Western blotting. Results Compared with MS group, the Angiotensin Ⅱ concentration in the valsartan group was significantly higher than that in the MS group [(77.1 ± 2.6) vs (59.8 ± 2.6) ng / g, P <0.01] (55.3 ± 5.2), (51.4 ± 2.6), (45.6 ± 2.7), (59.8 ± 2.6) ng / g, all P <0.05]. Compared with the normal control group, the expression of ACE mRNA and protein was up-regulated and the expression of ACE2 mRNA and protein were down-regulated in MS model rats. Valsartan and Astragalus could increase the expression of ACE2 mRNA and protein in MS rats and decrease the expression of ACE mRNA and protein [ACE2 protein: 1.47 ± 0.22, 1.12 ± 0.31, 1.12 ± 0.31, 0.19), high dose group (1.49 ± 0.20) than MS group (0.71 ± 0.10), all P <0.05; ACE protein: valsartan group (1.06 ± 0.21), Astragalus low dose group (1.75 ± 0.21) Group (1.43 ± 0.22), high dose group (1.15 ± 0.20) than MS group (2.09 ± 0.22), all P <0.05]. Conclusion The expression of ACE2 and the expression of ACE are increased in the heart of MS rats, and the levels of ACE2 and ACE are imbalanced. Gansu Astragalus can increase the expression of ACE2, decrease the expression of ACE and improve the ACE2 / ACE imbalance in the heart.