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为研究萘丁美酮的相对生物利用度，建立了用反相高效液相色谱法测定血浆中萘丁美酮的活性代谢物６－甲氧基－２－萘乙酸（ＭＮＡ）浓度的方法。实验结果表明，以萘普生作内标，甲醇∶０．０２ｍ ｏｌ／Ｌ醋酸盐缓冲液（ｐＨ３．０）为７４∶２６ 作流动相，用ＹＷＧ－Ｃ１８ 柱有良好的分离效果；血浆样品在ｐＨ３．０醋酸盐缓冲液存在下，用二氯甲烷萃取，回收率高，在２７０ｎｍ 波长下检测杂质峰少。该法标准曲线在０．５～６４ｍ ｇ／Ｌ范围内有良好线性，最低检测浓度为０．０２ｍ ｇ／Ｌ，萃取回收率为８８％ ～９４％ ，方法回收率为９６％ ～１０２％ ，日内相对标准差小于３．５％ ，日间相对标准差小于５％ 。具有快速简便，灵敏准确等特点，适用于萘丁美酮血药浓度测定及药代动力学和生物利用度研究用
To study the relative bioavailability of nabumetone, a method for the determination of 6-methoxy-2-naphthaleneacetic acid (MNA), the active metabolite of nabumetone, in plasma by reversed-phase high performance liquid chromatography was developed. The experimental results showed that naproxen was used as the internal standard, methanol: 0.02mol / L acetate buffer (pH3.0) was 74:26 as mobile phase, and YWG-C18 column had good separation effect. Plasma The sample was extracted with methylene chloride in the presence of pH 3.0 acetate buffer, with high recovery and less impurity peak at 270 nm. The standard curve of the method has a good linearity in the range of 0.5 ~ 64m g / L, the minimum detection concentration is 0.02m g / L, the extraction recovery is 88% ~ 94%, the recovery rate is 96% ~ 102% The relative standard deviations were less than 3.5% and the relative standard deviations were less than 5% during the day. Has the characteristics of quick and easy, sensitive and accurate, suitable for nabumetone blood concentration determination and pharmacokinetics and bioavailability